User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/23

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Follow the procedure as outlined by Dr.Hartings HERE.
  • Synthesize more HRP-Linked Au Nanoparticles at higher ratios.
  • Synthesize Myoglobin linked nanoparticles at standard ratios, dictated in yesterdays lab.
  • Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday.


Procedure

Making HRP and Myoglobin AuNP's

  • In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.

Oct 23 Solutions AC.png

  • After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.

UV-Vis of HRP and Pepsin AuNP's

Data