Difference between revisions of "User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/23"

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(Data)
(UV-Vis of HRP and Pepsin AuNP's)
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===UV-Vis of HRP and Pepsin AuNP's===
 
===UV-Vis of HRP and Pepsin AuNP's===
 +
*In this section 1 ml of each HRP and Pepsin Linked AuNP sample was taken and centrifuged for 30 minutes.
 +
*Next the supernatant was removed to be run using UV-Vis. The pellet was saved for each sample.
  
 
==Data==
 
==Data==

Revision as of 11:13, 28 October 2013

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Objective

  • Follow the procedure as outlined by Dr.Hartings HERE.
  • Synthesize more HRP-Linked Au Nanoparticles at higher ratios.
  • Synthesize Myoglobin linked nanoparticles at standard ratios, dictated in yesterdays lab.
  • Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday.


Procedure

Making HRP and Myoglobin AuNP's

  • In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.
  • Note that more HRP-Linked nanoparticles are being made because the original ratios made yesterday were large enough to induce fibers to form. We are looking to see at what concentration fibers start forming. Myoglobin nanoparticles are being made at the initial ratios used yesterday.

Oct 23 Solutions AC.png

  • After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.

UV-Vis of HRP and Pepsin AuNP's

  • In this section 1 ml of each HRP and Pepsin Linked AuNP sample was taken and centrifuged for 30 minutes.
  • Next the supernatant was removed to be run using UV-Vis. The pellet was saved for each sample.

Data

Oct 23 Pepsin UVVis.png

Oct 23 HRP UVVIS.png