Difference between revisions of "User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/23"

From OpenWetWare
Jump to: navigation, search
(Procedure)
(Making HRP and Myoglobin AuNP's)
Line 18: Line 18:
 
===Making HRP and Myoglobin AuNP's===
 
===Making HRP and Myoglobin AuNP's===
 
* In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.  
 
* In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.  
 +
*Note that more HRP-Linked nanoparticles are being made because the original ratios made yesterday were large enough to induce fibers to form. We are looking to see at what concentration fibers start forming. Myoglobin nanoparticles are being made at the initial ratios used yesterday.
 
[[Image:Oct_23_Solutions_AC.png]]
 
[[Image:Oct_23_Solutions_AC.png]]
*After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.  
+
*After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.
 +
 
 
===UV-Vis of HRP and Pepsin AuNP's===
 
===UV-Vis of HRP and Pepsin AuNP's===
  

Revision as of 12:09, 28 October 2013

BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Follow the procedure as outlined by Dr.Hartings HERE.
  • Synthesize more HRP-Linked Au Nanoparticles at higher ratios.
  • Synthesize Myoglobin linked nanoparticles at standard ratios, dictated in yesterdays lab.
  • Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday.


Procedure

Making HRP and Myoglobin AuNP's

  • In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.
  • Note that more HRP-Linked nanoparticles are being made because the original ratios made yesterday were large enough to induce fibers to form. We are looking to see at what concentration fibers start forming. Myoglobin nanoparticles are being made at the initial ratios used yesterday.

Oct 23 Solutions AC.png

  • After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.

UV-Vis of HRP and Pepsin AuNP's

Data