Difference between revisions of "User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24"

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==Objective==
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*Complete the procedure as outlined by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24|here]].<br.>
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*Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.>
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*Observe activity using UV-Vis after certain intervals of time.<br.>
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*At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.>
  
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==Procedure==
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*Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.>
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*Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.>
  
 
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==Data==
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*This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
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*It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.
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[[Image:Sept_24_Pep's.png]]
 
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*Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.
 
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Revision as of 11:21, 30 September 2013

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Objective

  • Complete the procedure as outlined by Dr.Hartings here.<br.>
  • Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.>
  • Observe activity using UV-Vis after certain intervals of time.<br.>
  • At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.>

Procedure

  • Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.>
  • Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.>

Data

  • This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
  • It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.

Sept 24 Pep's.png

  • Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.