Difference between revisions of "User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24"

From OpenWetWare
Jump to: navigation, search
(Data)
(Data)
Line 18: Line 18:
  
 
==Data==
 
==Data==
 +
*This graph represents the absorbacne spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
 +
*It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.
 
[[Image:Sept_24_Pep's.png]]
 
[[Image:Sept_24_Pep's.png]]
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 12:19, 30 September 2013

BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Complete the procedure as outlined by Dr.Hartings here.<br.>
  • Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.>
  • Observe activity using UV-Vis after certain intervals of time.<br.>
  • At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.>

Procedure

  • Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.>
  • Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.>

Data

  • This graph represents the absorbacne spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
  • It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.

Sept 24 Pep's.png