Difference between revisions of "User:Alexander.wong/sandbox"

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(Questions to ask the fluorometer Engineer on Friday, Aug 3)
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=Layout for New iGEM page=
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<div id="mainpage">
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{| style="width:100%; height:100px"
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| width="23%" rowspan=1 | [[Image:iGEM-logo.png|130px]]
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| width="25%"|
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| width="52%"| [[Image:ImperialCollege CentenaryLogo.png|280px]]
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|}
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{| cellspacing="5"
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|width="230px" class="blue" valign="top"|
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<!-- RESOURCES HEADER with edit link-->
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<h3><html>
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<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px">
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</html><font color=red>Breaking News !!</font></h3>
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<hr/>
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<!-- END INFORMATION HEADER with edit link-->
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* '''28 Aug 2007''' - 'Infector Modelling'
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* '''15 Aug 2007''' - 'We have vesicles!!'
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: '''News Archive link'''
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<h3><html>
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<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="INFORMATION" border="0">
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</html>At Base Camp</h3>
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<!-- END INFORMATION HEADER with edit link-->
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* '''[[IGEM:IMPERIAL/2007/Calendar | Calendar of Events]]'''
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* '''[[IGEM:IMPERIAL/2007/Literature | Resources]]'''
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* '''[[IGEM:IMPERIAL/2007/Ideas|Idea Discussions]]'''
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* '''[[IGEM:IMPERIAL/2007/Admin | Admin]]'''
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<h3><html>
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<img src="http://www.openwetware.org/images/thumb/0/04/Service_protocols.png/40px-Service_protocols.png" alt="INFORMATION" border="0" width="20px">
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</html>In the Wet Lab</h3>
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* '''[[IGEM:IMPERIAL/2007/Projects/Lab Notebook | Lab Notebook]]'''
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* '''[[IGEM:IMPERIAL/2007/Notebook/General Protocols | Protocols]]'''
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* '''[[IGEM:IMPERIAL/2007/DNA Constructs | DNA Constructs]]'''
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* '''Sequencing'''
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* '''[[IGEM:IMPERIAL/2007/Projects/Shopping Lists | Shopping List]]'''
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<h3><html>
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<img src="http://www.openwetware.org/images/3/35/Service_collaboration.png" alt="visitors" width="20px" border="0">
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</html>For our Visitors</h3>
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* [[IGEM:IMPERIAL/2007/Feedback | Feedback / Questions]]
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<center>
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'''Location of our visitors'''
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" />
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</center>
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|width="350px" class="white" valign="top"|
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<!-- INFORMATION HEADER with edit link-->
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<center>
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<h2>Imperial College London iGEM'07 Projects</h2>
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<big>'''[[IGEM:IMPERIAL/2007/Projects/In-Veso|Cell Free Systems]]'''</big>
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[[Image:IC07_CFS_cartoon.png|350px]]</center>
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<br>
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{|border="0" width="100%" align="center"
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! <big><html>
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<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px">
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</html>'''[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]] '''</big> !! <big><html>
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<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px">
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</html>'''[[IGEM:IMPERIAL/2007/Biofilm Detector|Infector Detector]]'''</big>
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|}<br>
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<h3><html>
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<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px">
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</html>Recent Changes</h3>
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<hr/>{{Special:Recentchanges/b=IGEM:IMPERIAL/2007&limit=10}}
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|width="220px" class="blue" valign="top"|
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<!-- PEOPLE HEADER with edit link-->
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<h3><html>
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<img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People">
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</html>The Team</h3>
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<hr/>
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<!-- END PEOPLE HEADER with edit link-->
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The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system.  In addition, we have a number of graduate student and faculty advisors.
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<font size="2">'''Undergraduates'''</font> {{hide|1=
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* [[User:James Chappell|James Chappell]]
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* [[User:Cheuk Ka Tong|Cheuk Ka Tong]]
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* [[User:Lucas_A._Hadjilucas|Lucas Hadjilucas]]
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* [[User:Jaroslaw Karcz|Jaroslaw Karcz]]
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* [[User:Anthony Lazzaro|Anthony Lazzaro]]
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* [[User:Peixuan Pey|Peixuan Pey]]
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* [[User:Dirk Van Swaay|Dirk van Swaay]]
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* [[User:Maira Tariq|Maira Tariq]]
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* [[User:Ben_Yi_Tew|Ben Yi Tew]]
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* [[User:alexander.wong|Alexander Wong]]
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}}
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<font size="2">'''Advisors'''</font> {{hide|1=
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* Prof Richard Kitney
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* Prof Paul Freemont
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* Duo Lu
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* Kirsten Jensen
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* Matthieu Bultelle
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* [[User:Vincent|Vincent Rouilly]]
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}}
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: '''Acknowledgments'''
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<h3><html>
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<img src="http://www.openwetware.org/images/4/45/GettingStarted_icon.png" alt="Entertainment" width="20px">
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</html>Entertainment</h3>
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<hr/>
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* '''[[IGEM:IMPERIAL/2007/Pictures | Pictures]]'''
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* '''[[IGEM:IMPERIAL/2007/Whiteboard | The Whiteboard]]'''
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* '''[[IGEM:IMPERIAL/2007/Quotes | Infamous Quotes]]'''
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* '''[[/Team Name and Logo | Discuss team name and logo]]'''
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<br>
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'''In partnership with'''
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[[Image:ImperialCollegeiGEM2007 Geneart Logo .png |left|200px|]]
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[http://www.geneart.com GeneART ]
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==Questions to ask the fluorometer Engineer on Friday, Aug 3==
 
==Questions to ask the fluorometer Engineer on Friday, Aug 3==
  

Revision as of 15:24, 30 August 2007

Layout for New iGEM page


IGEM-logo.png ImperialCollege CentenaryLogo.png

<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Breaking News !!


  • 28 Aug 2007 - 'Infector Modelling'
  • 15 Aug 2007 - 'We have vesicles!!'
News Archive link

<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="INFORMATION" border="0"> </html>At Base Camp


<html> <img src="http://www.openwetware.org/images/thumb/0/04/Service_protocols.png/40px-Service_protocols.png" alt="INFORMATION" border="0" width="20px"> </html>In the Wet Lab


<html> <img src="http://www.openwetware.org/images/3/35/Service_collaboration.png" alt="visitors" width="20px" border="0"> </html>For our Visitors


Location of our visitors <html> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" /> </a> </html>

Imperial College London iGEM'07 Projects

Cell Free Systems

IC07 CFS cartoon.png


<html>

<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px">

</html>Cell By Date
<html>

<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px"> </html>Infector Detector


<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Recent Changes


21 February 2018

     09:24 

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20 February 2018

     19:19  Lee:JC‎ (diff | hist) . . (+143). . Wooin Lee (talk | contribs) (Lab meeting schedule)

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<html> <img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>The Team


The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors.

Undergraduates

Advisors

  • Prof Richard Kitney
  • Prof Paul Freemont
  • Duo Lu
  • Kirsten Jensen
  • Matthieu Bultelle
  • Vincent Rouilly
Acknowledgments

<html> <img src="http://www.openwetware.org/images/4/45/GettingStarted_icon.png" alt="Entertainment" width="20px"> </html>Entertainment



In partnership with

ImperialCollegeiGEM2007 Geneart Logo .png

GeneART



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Questions to ask the fluorometer Engineer on Friday, Aug 3

See if our plate reader has the ability to measure what you want to measure.

  • What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
  • Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
  • Temperature-regulation?
  • How do we subtract background values from data and convert from absorbance to OD600?

Talk about using the plate reader and creating protocols.

  • Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
  • Any manufacturer protocols for plate reader usage?
  • Well plate rows and columns? How is data arranged sequentially with the wells?
  • What kind of plates you need to order and use for our experiments?
  • Lamp energy and Noise signal ratios??

Talk about processing data

  • Export format?
  • How to export data from fluorometer?
  • Built-in data analysis tools?
  • Technical Support.


Experiment 1: Fluorescence vs extracellular [GFP] molecules

Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.

Materials Required

Equipment

Reagents

Protocol

Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]

Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].

Materials Required

Equipment

Reagents

Protocol

Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]

Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].

Materials Required

Equipment

Reagents

Protocol