Difference between revisions of "User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08"

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(Objective)
(Bench Work)
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==Bench Work==
 
==Bench Work==
* Insert content here...
+
* 1000 ± 10μg of Au/mL of H<sub>2</sub>O stock solution
 +
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
 +
** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST  SRM 3121
 +
 
 +
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.
 +
 
 +
* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au from the 1000 ppm Au stock standard.
 +
**
 +
{| {{table}}
 +
| align="center" |'''ppm'''
 +
| align="center" |'''mL of 1000 ppm stock added'''
 +
|-
 +
| 5||0.25
 +
|-
 +
| 8||0.40
 +
|-
 +
| 10||0.50
 +
|-
 +
| 15||0.75
 +
|-
 +
| 20||1.00
 +
|-
 +
| 25||1.25
 +
|-
 +
| 30||1.50
 +
|-
 +
| 40||2.00
 +
|-
 +
|
 +
|}
  
 
==Data/Spectra==
 
==Data/Spectra==

Revision as of 11:49, 8 October 2012

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Objective

  • To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.

Bench Work

  • 1000 ± 10μg of Au/mL of H2O stock solution
    • from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
    • the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121

For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.

  • created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au from the 1000 ppm Au stock standard.
ppm mL of 1000 ppm stock added
5 0.25
8 0.40
10 0.50
15 0.75
20 1.00
25 1.25
30 1.50
40 2.00

Data/Spectra

  • Insert content here...

Notes

  • Insert content here...