Difference between revisions of "User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> CHEM571 </span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> CHEM571 </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Objective==
 
==Objective==
* Insert content here...
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* To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.
  
 
==Bench Work==
 
==Bench Work==
* Insert content here...
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* 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
 +
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
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** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST  SRM 3121
 +
 
 +
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm.
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For reference the molecular weight of gold is 197 g/mol.
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* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
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**from the 1000 ppm Au stock standard
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** using 50 mL volumetric flasks
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** all diluted in 1 M HCl
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{| {{table}}
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| align="center" |'''ppm'''
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| align="center" |'''mL of 1000 ppm stock added'''
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|-
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| 5||0.25
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|-
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| 8||0.40
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|-
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| 10||0.50
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|-
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| 15||0.75
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|-
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| 20||1.00
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|-
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| 25||1.25
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|-
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| 30||1.50
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|-
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| 40||2.00
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|-
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|
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|}
  
 
==Data/Spectra==
 
==Data/Spectra==
* Insert content here...
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* N/A - solutions will be used for AAS on 10/9/2012
  
 
==Notes==
 
==Notes==
* Insert content here...
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* N/A
  
 
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Latest revision as of 22:06, 26 September 2017

BDLlogo notext lr.png CHEM571 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective

  • To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.

Bench Work

  • 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
    • from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
    • the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121

For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm.

For reference the molecular weight of gold is 197 g/mol.

  • created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
    • from the 1000 ppm Au stock standard
    • using 50 mL volumetric flasks
    • all diluted in 1 M HCl
ppm mL of 1000 ppm stock added
5 0.25
8 0.40
10 0.50
15 0.75
20 1.00
25 1.25
30 1.50
40 2.00

Data/Spectra

  • N/A - solutions will be used for AAS on 10/9/2012

Notes

  • N/A