Tween 20: Difference between revisions
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* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | * polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | ||
* polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed. | * polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed. | ||
== Comments == | |||
* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html] | |||
== See also == | == See also == | ||
Revision as of 10:48, 14 October 2008
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Storage/Stability
Based on the SIGMA Tween20 data sheet [1]
- aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
- autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
- TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
- polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
- polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.
Comments
- "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background." [2]
