Difference between revisions of "Turner:Solutions/TnSeqLoTe"

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(New page: ==TnSeq LoTE - 50 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="center" style="backgro...)
 
 
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==TnSeq LoTE - 50 mL==
 
==TnSeq LoTE - 50 mL==
 
{| {{table}}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Component'''
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| align="center" style="background:#f0f0f0;"|'''50'''
| align="center" style="background:#f0f0f0;"|'''Final []'''
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| align="center" style="background:#f0f0f0;"|'''mL'''
| align="center" style="background:#f0f0f0;"|'''Stock []'''
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| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''Units'''
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| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''mL stock'''
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| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|''''''
 
|-
 
|-
| NaCl||2||5||M||20||||
+
| Component||Final []||Stock []||Units||mL stock||||
 
|-
 
|-
| Tris-HCl pH 7.4||0.01||1||M||0.5||||
+
| Tris-HCl pH 7.4||0.003||1||M||0.15||||
 
|-
 
|-
| EDTA pH 8||0.001||0.5||M||0.1||||
+
| EDTA pH 8||0.0002||0.5||M||0.02||||
 
|-
 
|-
 
|  
 
|  
 
|-
 
|-
| pH to||7.5||with||5M HCl||(approx)||0.5||drops
+
| pH to||7.5||with||5 M HCl||(approx)||0.2||drops
 
|-
 
|-
 
| sterilize by||f.s.||||||||||
 
| sterilize by||f.s.||||||||||

Latest revision as of 06:56, 31 October 2012

TnSeq LoTE - 50 mL

50 mL ' ' ' ' '
Component Final [] Stock [] Units mL stock
Tris-HCl pH 7.4 0.003 1 M 0.15
EDTA pH 8 0.0002 0.5 M 0.02
pH to 7.5 with 5 M HCl (approx) 0.2 drops
sterilize by f.s.
store at RT