Difference between revisions of "Turner:Solutions/GAMBITTEB"

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(New page: ==GAMBIT TEB== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="center" style="background:#f0f...)
 
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Latest revision as of 06:59, 31 October 2012

GAMBIT TEB

Component Final [] Stock [] Units mL stock ' '
Tris-HCl pH 7.4 0.02 1 M 0.2
NaCl 0.2 5 M 0.4
EDTA pH 8 0.001 0.5 M 0.02
DTT 0.002 0.1 M 0.2
Glycerol 10 50 % 2
Maltose 0.01 0.5 M 0.2
pH to 7.4 with 5N HCl (approx) 1 drop
sterilize by f.s.
store at 4C
Note: add 1 tablet cOmplete mini, EDTA-free to 10 mL buffer before use