Difference between revisions of "Triton X-100"

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(illustration)
(storage/stability)
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[[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]]
 
[[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]]
 
  
 
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
 
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
  
 
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== Storage/Stability ==
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from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
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* store at room temperature protected from bright light
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* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
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* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
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hydroxytoluene per 500 moles of detergent to prevent autoxidation
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== See also ==
 
== See also ==
 
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]
 
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]

Revision as of 10:32, 14 October 2008

Structure of Triton X-100

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

Storage/Stability

from the Pierce instructions manual [1]:

  • store at room temperature protected from bright light
  • susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
  • addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

See also

External links