Thermostable DNA ligase

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Revision as of 00:43, 14 April 2008 by Torsten Waldminghaus (talk | contribs) (New page: ==Purpose== *Thermostable DNA ligases can be added to a PCR reaction to ligate extended oligos. This is used for some mutagenesis methods. ==Procurement== *Examples for thermostable DNA l...)
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Purpose

  • Thermostable DNA ligases can be added to a PCR reaction to ligate extended oligos. This is used for some mutagenesis methods.

Procurement

  • Examples for thermostable DNA ligases are Taq ligase NEB and the Ampligase Epicentre.

Use

  • If you want to ligate extended oligos in a PCR reaction it is important to have them phosphorilated at the 5'-end. Oligos will only be ligated when they are assembled on a compementary strand.