Difference between revisions of "The mRNA Decay Resource:Protocols and Resources"

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[[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] <br>
 
[[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] <br>
 
[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br>
 
[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br>
[[Media:imblot.pdf|Immunoblotting]] <br>
+
[[Media:imblot.pdf|Immunoblotting]] <br><br>
 +
From Fred Sherman
 +
<biblio>
 +
#Paper1 pmid=12073320
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</biblio>
 +
<br><br>
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==General RNA Protocols==
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<biblio>
 +
#Paper1 pmid=18369981
 +
</biblio>

Revision as of 11:53, 17 June 2008

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

From Fred Sherman

  1. Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. PubMed ID:12073320 | HubMed [Paper1]



General RNA Protocols

  1. Bajak EZ and Hagedorn CH. Efficient 5' cap-dependent RNA purification : use in identifying and studying subsets of RNA. Methods Mol Biol. 2008;419:147-60. DOI:10.1007/978-1-59745-033-1_10 | PubMed ID:18369981 | HubMed [Paper1]