The BioBricks Foundation:Standards/Technical/Measurement

From OpenWetWare

Briefing on Measuring Promoters & RBSs

Video from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.

Promoter measurement test

Plate reader & FACS measurements of the promoter set at MIT

FACS of promoter set at Lawrence Berkeley



Notebook:Jason R. Kelly/Projects/BBF Meas Standards - Jason's lab notebook.

Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).

Resources about measuring gene expression

  1. Dyer BW, Ferrer FA, Klinedinst DK, and Rodriguez R. A noncommercial dual luciferase enzyme assay system for reporter gene analysis. Anal Biochem. 2000 Jun 15;282(1):158-61. DOI:10.1006/abio.2000.4605 | PubMed ID:10860516 | HubMed [1]