Difference between revisions of "Tetracycline"

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==Dosage==
 
==Dosage==
 
Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector.  The reduction in growth rate, described above was significant.
 
Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector.  The reduction in growth rate, described above was significant.
 +
 +
==Properties==
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*'''Molecular Weight''' - 480.9g
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 +
==References==
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*[[Molecular Cloning]]
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*[[Wikipedia:Tetracycline|Wikipedia Tetracycline page]]

Revision as of 12:54, 8 July 2005

Mode of Action

Tetracyclines (there are many) enter the cell by diffusion through OmpF porins. It inhibits growth by preventing codon-anticodon interactions during translation.

Resistance Mechanism

There are a number of resistance mechanisms, most require tetracycline antiporters. The most commonly used is the tetA(C) gene. The gene product of tetA(C) forms a pore in the inner membrane and exports one tet molecule for every proton imported. Molecular Cloning states that tet resistance has a number of adverse affects on the cell, chief among those being a reduction in growth rate and viability.

Dosage

Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector. The reduction in growth rate, described above was significant.

Properties

  • Molecular Weight - 480.9g

References