TempliPhi

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Overview

A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses rolling circle amplification.

Materials

Specific protocols

References

  1. Dean FB, Nelson JR, Giesler TL, and Lasken RS. Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res. 2001 Jun;11(6):1095-9. DOI:10.1101/gr.180501 | PubMed ID:11381035 | HubMed [Dean-GenomeRes-2001]
  2. Reagin MJ, Giesler TL, Merla AL, Resetar-Gerke JM, Kapolka KM, and Mamone JA. TempliPhi: A sequencing template preparation procedure that eliminates overnight cultures and DNA purification. J Biomol Tech. 2003 Jun;14(2):143-8. PubMed ID:14676313 | HubMed [Reagin-JBiomolTech-2003]
  3. TempliPhi FAQ [FAQ]
All Medline abstracts: PubMed | HubMed