Template:SSB-Protocols Col PCR

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Be sure to save some colonies on a block!

Do 4 colonies per transformation.
Pick colony and dab into PCR then dab into block.
Put block in incubator at 37C for 5-6hrs.
Use 33-50ul of PCR mix per colony.
Use PCR tubes!
Run colony PCR program based on fragment size.

Colony PCR Recipe:

H2O 41.5 41.5
10 mM dNTPs 1 1
10x Taq buffer 5 5
oligo 1 1 1
oligo 2 1 1
template 1 1
Taq polymerase 0.25 0.25