Template:SBB-Protocols Zymo1: Difference between revisions
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===Regular Zymo Cleanup=== | ===Regular Zymo Cleanup=== | ||
The following procedure removes the polymerase, dNTPs, buffer, and most of the oligonucleotides from a PCR reaction. It also will remove the buffer and restriction enzymes from a restriction digest reaction. | |||
#Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. | |||
#Transfer into the Zymo column (small clear guys) | |||
#spin through, discard waste. | |||
#Add 200 uL of Zymo Wash Buffer (which is basically 70% ethanol) | |||
#spin through, discard waste. | |||
#Add 200 uL of Zymo Wash Buffer | |||
#spin through, discard waste. | |||
#spin for 90 seconds, full speed to dry. | |||
#elute with water into a fresh Eppendorf tube, use the same volume of water as the volume of the original reaction | |||
Latest revision as of 09:09, 2 February 2012
Regular Zymo Cleanup
The following procedure removes the polymerase, dNTPs, buffer, and most of the oligonucleotides from a PCR reaction. It also will remove the buffer and restriction enzymes from a restriction digest reaction.
- Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction.
- Transfer into the Zymo column (small clear guys)
- spin through, discard waste.
- Add 200 uL of Zymo Wash Buffer (which is basically 70% ethanol)
- spin through, discard waste.
- Add 200 uL of Zymo Wash Buffer
- spin through, discard waste.
- spin for 90 seconds, full speed to dry.
- elute with water into a fresh Eppendorf tube, use the same volume of water as the volume of the original reaction
