Template:SBB-Protocols Enz3: Difference between revisions
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8.5uL of eluted PCR product | 8.5uL of eluted PCR product | ||
1uL of NEB Buffer 2 | 1uL of NEB Buffer 2 | ||
1uL BglII | |||
*Incubate at 37 degrees on the thermocycler for 1hr | *Incubate at 37 degrees on the thermocycler for 1hr | ||
*Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees | *Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees | ||
*Proceed with remainder of Zymo gel purification protocol | *Proceed with remainder of Zymo gel purification protocol | ||
Latest revision as of 16:54, 23 June 2010
BglII Digest of EIPCR Products
For EIPCR products, you will only digest a portion of your purified PCR product. This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.
- Set up the following reaction:
8.5uL of eluted PCR product 1uL of NEB Buffer 2 1uL BglII
- Incubate at 37 degrees on the thermocycler for 1hr
- Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
- Proceed with remainder of Zymo gel purification protocol
