Template:ASUBME103 f2014 L6: Difference between revisions
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<!-- Instructions: add the name of your team's company and/or the brand name of your product here --> | |||
=LAB 6 WRITE-UP= | =LAB 6 WRITE-UP= | ||
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'''TinkerCAD'''<br> | '''TinkerCAD'''<br> | ||
<!-- Instructions: Write a short summary (up to five sentences) of the TinkerCAD tool and how you used it during the Computer-Aided Design lab --> | |||
'''Our Design'''<br> | '''Our Design'''<br> | ||
<!-- Instructions: Show an image of your TinkerCAD design here --> | |||
<!-- Instructions: Under the image, write a short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design? --><br> | |||
<br> | <br> | ||
==Feature 1: Disease SNP-Specific Primers== | ==Feature 1: Disease SNP-Specific Primers== | ||
<!-- Instructions: This part of your report will come from the exercises you did in PCR Lab B. --> | |||
'''Background on the disease-associated mutation'''<br> | '''Background on the disease-associated mutation'''<br> | ||
<!-- Instructions: Use the answers from questions 3 - 7 to compose, in your own words, a paragraph about rs16991654 --> | |||
'''Primer design'''<br> | '''Primer design'''<br> | ||
<!-- Type-in the sequence of the forward primer and reverse primer in the appropriate spaces --> | |||
* Disease SNP-specific Forward Primer: | * Disease SNP-specific Forward Primer: Instructions: type the sequence of the forward primer]'' | ||
* Reverse Primer: ''[Instructions: type the sequence of the reverse primer]'' | * Reverse Primer: ''[Instructions: type the sequence of the reverse primer]'' | ||
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==Feature 2: Consumables Kit== | ==Feature 2: Consumables Kit== | ||
<!-- Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | |||
<!-- Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | |||
==Feature 3: Hardware - PCR Machine & Fluorimeter== | ==Feature 3: Hardware - PCR Machine & Fluorimeter== |
Revision as of 15:29, 16 August 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD
Feature 1: Disease SNP-Specific PrimersBackground on the disease-associated mutation
Primer design
How the primers work: [Instructions: explain what makes the primers disease-sequence specific. In other words, explain why the primers will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele.]
Feature 2: Consumables KitFeature 3: Hardware - PCR Machine & Fluorimeter[Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.]
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]
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