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1. How to obtain serum from a blood sample

To obtain serum, the coagulated blood is left to clot at room temperature for approximately 15 to 30 minutes. After it is completely clotted, it is rimmed using an applicator stick and then centrifuged for approximately 5-10minutes at 2500 revolutions per minute. Then the supernatant fluid is separated.

Read more: http://wiki.answers.com/Q/How_to_obtain_serum_from_a_blood_sample#ixzz1Ol5IaaS1