Difference between revisions of "Talk:Synthetic Biology:BioBricks/Standard FACS protocol"

From OpenWetWare
Jump to: navigation, search
 
 
Line 1: Line 1:
 
*'''[[User:Rshetty|RS]] 12:35, 23 March 2006 (EST)''': I posted Barry's standard protocols from ages ago for Pete Carr.  This is severely in need of an update.
 
*'''[[User:Rshetty|RS]] 12:35, 23 March 2006 (EST)''': I posted Barry's standard protocols from ages ago for Pete Carr.  This is severely in need of an update.
 +
 +
# Use defined rich media with glycerol as the carbon source wherever possible (see [[Neidhardt_EZ_Rich_Defined]]).
 +
#Use beads to calibrate runs against one another and control for machine variation and PMT settings etc.
 +
#If possible use MG1655 as the background strain because at least its sequence is known in contrast to a lot of other strains whose exact genotype can be either fuzzy or wrong.
 +
#Move to using a near single copy plasmid for all characterization.  See [[Synthetic_Biology:Vectors/Single_copy_plasmid]].

Latest revision as of 10:54, 23 March 2006

  • RS 12:35, 23 March 2006 (EST): I posted Barry's standard protocols from ages ago for Pete Carr. This is severely in need of an update.
  1. Use defined rich media with glycerol as the carbon source wherever possible (see Neidhardt_EZ_Rich_Defined).
  2. Use beads to calibrate runs against one another and control for machine variation and PMT settings etc.
  3. If possible use MG1655 as the background strain because at least its sequence is known in contrast to a lot of other strains whose exact genotype can be either fuzzy or wrong.
  4. Move to using a near single copy plasmid for all characterization. See Synthetic_Biology:Vectors/Single_copy_plasmid.