Difference between revisions of "Talk:Shreffler:CFSE Labeling"

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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 
#List troubleshooting tips here.   
 
#List troubleshooting tips here.   
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
 
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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== CFSE toxicity ==
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I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment
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*'''[[User:Wayne G. Shreffler|Wayne G. Shreffler]] 22:28, 19 May 2009 (EDT)''':

Latest revision as of 19:45, 19 May 2009

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

CFSE toxicity

I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment