Talk:Knight:Purification of His-tagged proteins/Denaturing

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Sample results

Denaturing PAGE of ~13kD His tagged protein purified via this method.

Lane order:

  1. SeeBlue Plus2 Pre-Stained Standard
  2. Cleared lysate
  3. Flow through (after column binding)
  4. Flow through (after column binding, second time)
  5. Wash 1
  6. Wash 2
  7. Eluted protein
  8. Cleared lysate from cells not expressing a His-tagged protein
  9. Flow through (after column binding, second time) from cells not expressing a His-tagged protein
  10. Wash 2 from cells not expressing a His-tagged protein