Talk:Haynes:Gibson Assembly

From OpenWetWare
Revision as of 15:14, 5 January 2013 by Karmella Haynes (talk | contribs)
Jump to: navigation, search
  • ---Karmella 22:30, 26 November 2012 (EST): Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.
  • ---Karmella 18:14, 5 January 2013 (EST): Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)