Talk:Endy:Preparing Antibiotic Stocks: Difference between revisions
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(New page: Chloramphenicol * Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) Are stringent and relaxed...) |
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Are stringent and relaxed the wrong way around...? | Are stringent and relaxed the wrong way around...? | ||
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them. |
Revision as of 06:08, 17 January 2008
Chloramphenicol
* Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
Are stringent and relaxed the wrong way around...?
- Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.