Difference between revisions of "Talk:CH391L/S12/PCR and advanced PCR techniques"

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:*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique!
 
:*'''[[User:Yi Kou|Yi Kou]] 18:24, 6 February 2012 (EST)''':Really nice technique!
 
*[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
 
*[[User:Razan Alnahhas|Razan Alnahhas]] 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
 
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*'''[[User:Ben Slater|Ben Slater]] 12:28, 10 February 2012 (EST)''': Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity?
  
  

Revision as of 09:28, 10 February 2012

  • Brian Renda 15:47, 6 February 2012 (EST): Another cool PCR variant is called RPA (recombinase polymerase amplification), it is isothermal, finished in about 10-15 minutes, and the ingredients can be dried and stored at room temperature [1].
  • Yi Kou 18:24, 6 February 2012 (EST):Really nice technique!
  • Razan Alnahhas 23:12, 9 February 2012 (EST): Has PCR mediated DNA shuffling been successfully used in directed evolution, and are there only certain sequences that this technique can work on?
  • Ben Slater 12:28, 10 February 2012 (EST): Hi Yi, I noticed you mentioned a few things about Phusion being both more accurate and faster than Taq. So what is the downside? Price? Does it have any different thermal sensitivity?


References

  1. Piepenburg O, Williams CH, Stemple DL, and Armes NA. DNA detection using recombination proteins. PLoS Biol. 2006 Jul;4(7):e204. DOI:10.1371/journal.pbio.0040204 | PubMed ID:16756388 | HubMed [Piepenburg2006]