Talk:CH391L/S12/Origins of Replication: Difference between revisions
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The section "Commonly used BioBrick plasmid backbones" has links to [http://partsregistry.org/Plasmid_backbones/Assembly Assembly] and [http://partsregistry.org/Plasmid_backbones/Operation Operation]. These are very similar and suggest pMB1/ColE1, pSC101, P15A, F, and P1. | The section "Commonly used BioBrick plasmid backbones" has links to [http://partsregistry.org/Plasmid_backbones/Assembly Assembly] and [http://partsregistry.org/Plasmid_backbones/Operation Operation]. These are very similar and suggest pMB1/ColE1, pSC101, P15A, F, and P1. | ||
The section "Plasmid backbones for advanced users" has almost nothing in | The section "Plasmid backbones for advanced users" has almost nothing in it, except for a brief mention of pMB1/ColE1. | ||
The section "Plasmid backbones that comply with other assembly standards" has a link called | The section "Plasmid backbones that comply with other assembly standards" has a link called | ||
Revision as of 08:34, 8 March 2012
- Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the origin on the iGEM plasmid that BioBricks are distributed in?
- Adam Meyer 10:34, 8 March 2012 (EST):There doesn't seem to be a standard. This a page called Plasmid Backbones with several sections:
The section "Commonly used BioBrick plasmid backbones" has links to Assembly and Operation. These are very similar and suggest pMB1/ColE1, pSC101, P15A, F, and P1.
The section "Plasmid backbones for advanced users" has almost nothing in it, except for a brief mention of pMB1/ColE1.
The section "Plasmid backbones that comply with other assembly standards" has a link called Other assembly standard plasmid backbones that list a few labs. The are four labs that meet the standards for a backbone, but one one of them (Berkeley) lists their oris (They use pSC101 and RK2/oriV).
"Miscellaneous other plasmid backbones" mentions pMB1/ColE1, P15A, F, and R6K.
If I were a iGEMer and wanted to build constructs, I would use a pMB1/ColE1 backbone (e.g. pUC, pET, pBR322 etc). Their higher copy allows for easier cloning and sequencing. P15A backbones (e.g. pACYC, pBad) are next best choice, if you need a two-plasmid system, but their copy numbers are lower, so they are kind of a pain for routine cloning.
- Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the highest known copy number of any plasmid?
