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|−|Suggestion 1: On day 2 we ran gels of the cell lysates from induced and uninduced e coli. It might be a good idea to load a sample of recombinant beta-gal on these gels to make it more clear where the induced beta-gal might be running on the gel. |+|
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|−|Suggestion 2: On day 3 we purified beta-gal from our cell lysates and measured activity of the purified material. It might be fun for the students to measure activity of the supernatant as well . This would give them the opportunity to think about the efficiency of their purification and some steps that they might take to improve efficiency if it turns out their recovery was less than optimal. |+|
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Latest revision as of 02:40, 7 May 2006
- RS 17:06, 5 May 2006 (EDT): Make a version of OpenWetWare:Getting started specifically geared towards BE.109. (Reshma should do this.)
- yeem 17:25, 5 May 2006 (EDT): I'd be willing to help.
- RS 17:46, 5 May 2006 (EDT): Cool. Will be in touch.
yeem 21:59, 6 May 2006 (EDT)' Made a couple changes. Didn't know what to do for "posting your data"; should we be encouraging people to post on their user pages?
- Natalie I would prefer to have data posted on the discussion page associated with the day in lab, believing it will keep the information better organized for the class overall as well as allowing folks to better compare their data to the data that others collect. Happy to hear other thoughts though. I also wanted to thank you both for working on this now.