Difference between revisions of "Talk:Agarose gel loading buffer"

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(Do you know a recipe for a direct loading Taq PCR buffer (with dyes)?)
(No difference)

Revision as of 08:28, 24 April 2008

Hi, I wonder if anyone has a recipe for the (Taq) PCR buffers sold by some companies, with which the PCR reaction can be directly loaded on the gel. What do they use as density reagent? Has anyone tried to make her own direct-loading buffer yet...and tested the efficiency of amplification in this buffer compared to standard taq buffer recipies? --Mathias Klode 11:28, 24 April 2008 (EDT)