Talk:20.109(S14):Diagnostic primer design (Day2): Difference between revisions
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|Silver | |Silver | ||
| | | CAC CAG GTT GAT TCT GCC TGA C | ||
| | | TTC ACC ATA GAC ACT ACT GGA GC | ||
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| | | | ||
Revision as of 14:40, 12 February 2014
T/R Section
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Red | CAC CAG GTT GAT ACT GCC TGA C | CCT ATC CCG AAC CAA ACC CTG | bp 1 | bp 260 | EH, SSU rRNA |
| Green | |||||
| Pink | ACC AGG TTG ATT CTG CCT GAC | TCT CTC ACG CTC CCT CTC C | bp1/bp2 | bp252/bp290 | EH/VC SSU rRna |
Specificity
| Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Yellow | |||||
| Blue | GATTCTGCCTGACGTAGATGC | CTGCTGGCACCAAACTTGC | 10 bp | 408 bp | E. hellem (AF118143) |
| Orange |
W/F Section
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target site (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Pink | ACC AGG TTG ATT CTG CTG ACG | TTGCGAGCATACTATCCCCAG | bp 1/2 | bp 741/768 | VC/EH SSU rRNA |
| Red | ACC AGG TTG ATT CTG CCT GAC | ATA TTA CCG CGG CTG CTG | bp 1/2 | bp 380/421 | VC/EH small subunit rRNA |
| Orange | |||||
Specificity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target site (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| White | |||||
| Silver | CAC CAG GTT GAT TCT GCC TGA C | TTC ACC ATA GAC ACT ACT GGA GC | |||
| Yellow | CAC CAG GTT GAT TCT GCC TGA C | CCA TCC ATT TCC AAC GAC CAT G | 1 | 947 | EH |
| Purple | GTCTGGCTGTGTAGATAACTACG | ||||
| Green |
