Difference between revisions of "Talk:20.109(S14):Cell preparation for DNA repair assays (Day5)"

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Concentrations of TA Prepped DNA
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==Concentrations of Student Prepped DNA==
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 +
Note: All TA prepped DNA will be at 50 ng/μL.
  
 
{| border="1"
 
{| border="1"
 
! Cut topology
 
! Cut topology
 
! Group 1
 
! Group 1
! Concentration
+
! Concentration (ng/uL)
 
! Group 2
 
! Group 2
! Concentration
+
! Concentration (ng/uL)
 
! (Group 3)
 
! (Group 3)
! (Concentration)
+
! (Concentration (ng/uL))
 
|-
 
|-
 
| Sticky ends, 5' overhangs  
 
| Sticky ends, 5' overhangs  
 
| Orange Team (TR)
 
| Orange Team (TR)
|
+
| 27.0
 
| Red Team (WF)
 
| Red Team (WF)
 +
| 18.7
 
|
 
|
 
|
 
|
Line 19: Line 22:
 
| Sticky ends, 3' overhangs  
 
| Sticky ends, 3' overhangs  
 
|Blue Team (TR)
 
|Blue Team (TR)
 +
|28.5
 
|Purple Team (WF)
 
|Purple Team (WF)
 +
|11.0
 +
|
 
|
 
|
 
|-
 
|-
 
| Blunt  
 
| Blunt  
 
|Yellow Team (TR)
 
|Yellow Team (TR)
 +
|21.4
 
|Yellow Team (WF)
 
|Yellow Team (WF)
 +
|27.3
 
|Blue Team (WF)
 
|Blue Team (WF)
 +
|34.0
 
|-
 
|-
 
| Hybrid: 5' sticky upstream, blunt downstream  
 
| Hybrid: 5' sticky upstream, blunt downstream  
 
|Pink Team (TR)
 
|Pink Team (TR)
 +
|34.9
 
|Green Team (WF)
 
|Green Team (WF)
 +
|34.6
 +
|
 
|
 
|
 
|-
 
|-
 
| Hybrid: 3' sticky upstream, blunt downstream  
 
| Hybrid: 3' sticky upstream, blunt downstream  
 
|Red Team (TR)
 
|Red Team (TR)
 +
|21.8
 
|Pink Team (WF)
 
|Pink Team (WF)
 +
|51.8
 +
|
 
|
 
|
 
|-
 
|-
 
| Sticky ends with sequence mismatch  
 
| Sticky ends with sequence mismatch  
 
|Green Team (TR)
 
|Green Team (TR)
 +
|29.7
 
|White Team (WF)
 
|White Team (WF)
 +
|18.1
 +
|
 
|
 
|
 
|-
 
|-
 
| Sticky ends with sequence and topology mismatch
 
| Sticky ends with sequence and topology mismatch
 
|BA Orange Team (WF)
 
|BA Orange Team (WF)
 +
|12.6
 
|not so BA Silver Team (WF)
 
|not so BA Silver Team (WF)
 +
|33.7
 +
|
 
|
 
|
 
|-
 
|-
 
|}
 
|}

Latest revision as of 13:00, 28 March 2014

Concentrations of Student Prepped DNA

Note: All TA prepped DNA will be at 50 ng/μL.

Cut topology Group 1 Concentration (ng/uL) Group 2 Concentration (ng/uL) (Group 3) (Concentration (ng/uL))
Sticky ends, 5' overhangs Orange Team (TR) 27.0 Red Team (WF) 18.7
Sticky ends, 3' overhangs Blue Team (TR) 28.5 Purple Team (WF) 11.0
Blunt Yellow Team (TR) 21.4 Yellow Team (WF) 27.3 Blue Team (WF) 34.0
Hybrid: 5' sticky upstream, blunt downstream Pink Team (TR) 34.9 Green Team (WF) 34.6
Hybrid: 3' sticky upstream, blunt downstream Red Team (TR) 21.8 Pink Team (WF) 51.8
Sticky ends with sequence mismatch Green Team (TR) 29.7 White Team (WF) 18.1
Sticky ends with sequence and topology mismatch BA Orange Team (WF) 12.6 not so BA Silver Team (WF) 33.7