Talk:20.109(S13):Context-setting and primer design (Day1): Difference between revisions
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When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered. | When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered. | ||
I will name your primers by the approximate base-pair that they target in ''V corneae'' or ''E hellem'', so please share this information as well. | |||
===Sensitivity=== | ===Sensitivity=== | ||
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{| border="1" | {| border="1" | ||
|Team color | |Team color | ||
|Forward primer | |Forward (F) primer | ||
|Reverse primer | |Reverse (R) primer | ||
|Target site (e.g. bp 60), F | |||
|Target side (e.g., bp 300), R | |||
|Is bp # for VC or EH? Which gene? | |||
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| Grey (Platinum) :) | |||
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|Green | |||
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|Pink | |||
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|Yellow | |||
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{| border="1" | {| border="1" | ||
|Team | |Team Color | ||
|Forward primer | |Forward primer | ||
|Reverse primer | |Reverse primer | ||
|Target site (e.g. bp 60), F | |||
|Target side (e.g., bp 300), R | |||
|Is bp # for VC or EH? Which gene? | |||
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Revision as of 15:02, 7 February 2013
T/R section
Please sign up for one of the design challenges -- four teams per challenge -- right away.
When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered.
I will name your primers by the approximate base-pair that they target in V corneae or E hellem, so please share this information as well.
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Grey (Platinum) :) | |||||
| Green | |||||
| Pink | |||||
| Yellow |
Specificity
| Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Purple | |||||
| Red | |||||
| Orange | |||||
| Blue |
W/F section
Please sign up for one of the design challenges -- four teams per challenge -- right away.
When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered.
I will name your primers by the approximate base-pair that they target in V corneae or E hellem, so please share this information as well.
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Grey (Platinum) :) | |||||
| Green | |||||
| Pink | |||||
| Yellow |
Specificity
| Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
