Talk:20.109(S13):Characterize protein expression (Day7)

From OpenWetWare
Revision as of 11:40, 10 April 2013 by AgiStachowiak (talk | contribs) (SDS-PAGE images)
Jump to: navigation, search

SDS-PAGE analysis tips

You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!

If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.

SDS-PAGE images


File:S13 M2D6 TR Red.JPG
T/R Gel, Red group
File:S13 M2D6 TR Orange.JPG
T/R Gel, Orange group
File:S13 M2D6 TR Yellow.JPG
T/R Gel, Yellow group
File:S13 M2D6 TR Green.JPG
T/R Gel, Green group
File:S13 M2D6 TR Blue.JPG
T/R Gel, Blue group
File:S13 M2D6 TR Purple.JPG
T/R Gel, Purple group
File:S13 M2D6 TR Platinum.JPG
T/R Gel, Platinum group