Difference between revisions of "Talk:20.109(S13):Characterize protein expression (Day7)"

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(New page: ==SDS-PAGE analysis tips== You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and [http://www.bioinformatics.org/sms/prot_mw.html this link...)
 
(SDS-PAGE images)
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==SDS-PAGE images==
 
==SDS-PAGE images==
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===T/R===
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[[Image:S13_M2D6_TR_Red.JPG | right |thumb|250px | T/R Gel, Red group]]
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[[Image:S13_M2D6_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]]
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[[Image:S13_M2D6_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group]]
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[[Image:S13_M2D6_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group]]
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[[Image:S13_M2D6_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group]]
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[[Image:S13_M2D6_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]]
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[[Image:S13_M2D6_TR_Platinum.JPG | left |thumb|250px | T/R Gel, Platinum group]]
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===W/F===

Revision as of 11:40, 10 April 2013

SDS-PAGE analysis tips

You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!

If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.

SDS-PAGE images

T/R

File:S13 M2D6 TR Red.JPG
T/R Gel, Red group
File:S13 M2D6 TR Orange.JPG
T/R Gel, Orange group
File:S13 M2D6 TR Yellow.JPG
T/R Gel, Yellow group
File:S13 M2D6 TR Green.JPG
T/R Gel, Green group
File:S13 M2D6 TR Blue.JPG
T/R Gel, Blue group
File:S13 M2D6 TR Purple.JPG
T/R Gel, Purple group
File:S13 M2D6 TR Platinum.JPG
T/R Gel, Platinum group

W/F