Talk:20.109(S13):Characterize protein expression (Day7): Difference between revisions
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(New page: ==SDS-PAGE analysis tips== You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and [http://www.bioinformatics.org/sms/prot_mw.html this link...) |
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==SDS-PAGE images== | ==SDS-PAGE images== | ||
===T/R=== | |||
[[Image:S13_M2D6_TR_Red.JPG | right |thumb|250px | T/R Gel, Red group]] | |||
[[Image:S13_M2D6_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]] | |||
[[Image:S13_M2D6_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group]] | |||
[[Image:S13_M2D6_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group]] | |||
[[Image:S13_M2D6_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group]] | |||
[[Image:S13_M2D6_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]] | |||
[[Image:S13_M2D6_TR_Platinum.JPG | left |thumb|250px | T/R Gel, Platinum group]] | |||
===W/F=== | |||
Revision as of 12:40, 10 April 2013
SDS-PAGE analysis tips
You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.
