Difference between revisions of "Talk:20.109(F13): Mod 2 Day 6 Analysis & Planning 2"

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(WF Blots)
 
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===TR Blots===
+
===TR Blots===
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]]
+
'''TR Intensities'''
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]]
+
Defaults
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]]
+
*EGFR - 700:5, 800:3
 +
*GAPDH - 700:2, 800:5
 +
*Akt - 700:2, 800: 6
 +
*ERK - 700:1, 800:3
 +
*Pink STAT3 - 700:7, 800:9
 +
*Pink EGFR - 700:7, 800:3
 +
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]]  
 +
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]]  
 +
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]]  
 
[[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]]
 
[[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]]
 
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]]
 
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]]
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[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]]
 
[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]]
 
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]]
 
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]]
 +
[[Image:Green_TR_pAkt2.tif|thumb|left|300px|Green pAkt_2]]
 +
[[Image:Green_TR_GAPDH2.tif|thumb|left|300px|Green GAPDH_2]]
 +
[[Image:Green_TR_totalAkt2.tif|thumb|left|300px|Green Total Akt 2]]
 
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]]
 
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]]
 
[[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]]
 
[[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]]
 
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]]
 
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]]
 +
[[Image:Orange_TR_pAkt2.tif|thumb|left|300px|Orange pAkt_2]]
 +
[[Image:Orange_TR_GAPDH2.tif|thumb|left|300px|Orange GAPDH_2]]
 +
[[Image:Orange_TR_totalAkt2.tif|thumb|left|300px|Orange Total Akt 2]]
 
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]]
 
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]]
 
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]]
 
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]]
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[[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]]
 
[[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]]
 
[[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]]
 
[[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]]
 +
[[Image:Green_EGFR.tif|thumb|left|300px|Green EGFR]]
 +
[[Image:Green_pAkt.tif|thumb|left|300px|Green pAkt]]
 +
[[Image:Green_GAPDH.tif|thumb|left|300px|Green GAPDH]]
 +
[[Image:Plat_EGFR.tif|thumb|left|300px|Plat EGFR]]
 +
[[Image:Plat_pAkt.tif|thumb|left|300px|Plat pAkt]]
 +
[[Image:Plat_GAPDH.tif|thumb|left|300px|Plat GAPDH]]
 +
[[Image:Plat_TR_pAkt2.tif|thumb|left|300px|Plat pAkt_2]]
 +
[[Image:Plat_TR_GAPDH2.tif|thumb|left|300px|Plat GAPDH_2]]
 +
[[Image:Plat_TR_totalAkt2.tif|thumb|left|300px|Plat Total Akt 2]]
 +
<br style="clear:both;"/>
  
 
+
===WF Blots===
 
+
'''WF Intensities'''
===Intensities===
+
ERK
*Defaults
+
*White: 2/3
 
+
*Pink: 1.5/3
ERK: 700 - 1.0, 800 - 3.0
+
*Green: 2.5/3
 
+
STAT3
EGFR: 700 - 5.0, 800 - 3.0
+
*Red: 6/7.5
 
+
*Blue: 6/8
Akt: 700 - 2.0, 800 - 6.0
+
GAPDH (All Teams)
 
+
* 2/5
GAPDH: 700 - 2.0, 800 - 5.0
+
[[Image:Yellow_WF_EGFR.tif|thumb|center|300px|Yellow EGFR]]
 
+
[[Image:Yellow_WF_pAkt.tif|thumb|center|300px|Yellow pAkt]]
*Changes (if necessary)
+
[[Image:Yellow_WF_GAPDH.tif|thumb|center|300px|Yellow GAPDH]]
 
+
[[Image:Yellow_WF_totalAkt.tif|thumb|center|300px|Yellow total Akt]]
Pink EGFR: 700 - 7.0, 800 - 3.0
+
*Note, Yellow blots are in the reverse order.
 
+
[[Image:Purple_WF_EGFR.tif|thumb|center|300px|Purple EGFR]]
Pink STAT: 700 - 7.0, 800 - 9.0
+
[[Image:Purple_pAkt.tif|thumb|center|300px|Purple pAkt]]
 +
[[Image:Purple_WF_GAPDH.tif|thumb|center|300px|Purple GAPDH]]
 +
[[Image:Orange_WF_EGFR.tif|thumb|center|300px|Orange EGFR]]
 +
[[Image:Orange_WF_GAPDH.tif|thumb|center|300px|Orange GAPDH]]
 +
[[Image:Red_WF_EGFR.tif|thumb|center|300px|Red EGFR]]
 +
[[Image:Red_WF_STAT3_2.tif|thumb|center|300px|Red STAT3]]
 +
[[Image:Red_WF_GAPDH.tif|thumb|center|300px|Red GAPDH]]
 +
[[Image:Blue_WF_EGFR.tif|thumb|center|300px|Blue EGFR]]
 +
[[Image:Blue_WF_STAT3_2.tif|thumb|center|300px|Blue STAT3]]
 +
[[Image:Blue_WF_GAPDH.tif|thumb|center|300px|Blue GAPDH]]
 +
[[Image:Pink_WF_EGFR.tif|thumb|center|300px|Pink EGFR]]
 +
[[Image:Pink_WF_ERK.tif|thumb|center|300px|Pink ERK]]
 +
[[Image:Green_WF_EGFR.tif|thumb|center|300px|Green EGFR]]
 +
[[Image:Green_WF_ERK.tif|thumb|center|300px|Green ERK]]
 +
[[Image:White_WF_EGFR.tif|thumb|center|300px|White EGFR]]
 +
[[Image:White_WF_Erk.tif|thumb|center|300px|White ERK]]

Latest revision as of 09:17, 6 November 2013

TR Blots

TR Intensities Defaults

  • EGFR - 700:5, 800:3
  • GAPDH - 700:2, 800:5
  • Akt - 700:2, 800: 6
  • ERK - 700:1, 800:3
  • Pink STAT3 - 700:7, 800:9
  • Pink EGFR - 700:7, 800:3

File:Yellow TR EGFR.tif File:Yellow TR ERK.tif File:Purple TR EGFR.tif File:Purple TR ERK.tif File:White TR EGFR.tif File:White TR ERK.tif File:Green TR EGFR.tif File:Green TR pAkt.tif File:Green TR GAPDH.tif File:Green TR pAkt2.tif File:Green TR GAPDH2.tif File:Green TR totalAkt2.tif File:Orange TR EGFR.tif File:Orange pAkt.tif File:Orange TR GAPDH.tif File:Orange TR pAkt2.tif File:Orange TR GAPDH2.tif File:Orange TR totalAkt2.tif File:Pink TR EGFR2.tif File:Pink TR STAT3.tif File:Pink TR GAPDH.tif File:Blue TR EGFR.tif File:Blue TR STAT3.tif File:Blue TR GAPDH.tif File:Red TR EGFR.tif File:Red TR STAT3.tif File:Red TR GAPDH.tif File:Green EGFR.tif File:Green pAkt.tif File:Green GAPDH.tif File:Plat EGFR.tif File:Plat pAkt.tif File:Plat GAPDH.tif File:Plat TR pAkt2.tif File:Plat TR GAPDH2.tif File:Plat TR totalAkt2.tif

WF Blots

WF Intensities ERK

  • White: 2/3
  • Pink: 1.5/3
  • Green: 2.5/3

STAT3

  • Red: 6/7.5
  • Blue: 6/8

GAPDH (All Teams)

  • 2/5

File:Yellow WF EGFR.tif File:Yellow WF pAkt.tif File:Yellow WF GAPDH.tif File:Yellow WF totalAkt.tif

  • Note, Yellow blots are in the reverse order.

File:Purple WF EGFR.tif File:Purple pAkt.tif File:Purple WF GAPDH.tif File:Orange WF EGFR.tif File:Orange WF GAPDH.tif File:Red WF EGFR.tif File:Red WF STAT3 2.tif File:Red WF GAPDH.tif File:Blue WF EGFR.tif File:Blue WF STAT3 2.tif File:Blue WF GAPDH.tif File:Pink WF EGFR.tif File:Pink WF ERK.tif File:Green WF EGFR.tif File:Green WF ERK.tif File:White WF EGFR.tif File:White WF Erk.tif