Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

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Revision as of 12:07, 18 September 2013 by AgiStachowiak (talk | contribs) (Pre-purification gels (for reference/posterity))
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Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.


T/R lab

Here is your recovery gel: TR F1320109 M1D3 recoverygel.BMP

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

W/F lab

Recovery gel

Pre-purification gels (for reference/posterity)

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Red Group
Orange Group
Yellow Group
Green Group
Blue Group
Pink Group
Purple Group
White Group