Difference between revisions of "Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis"

From OpenWetWare
Jump to: navigation, search
(New page: === Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. === left right<br> <br style=...)
 
Line 1: Line 1:
=== Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. ===
+
== Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. ==
 
[[Image:FragsMod1.jpg|left]]
 
[[Image:FragsMod1.jpg|left]]
 
[[Image:Excisedfrag.jpg|right]]<br>
 
[[Image:Excisedfrag.jpg|right]]<br>
 
<br style="clear:both" />
 
<br style="clear:both" />
  
=T/R lab=
+
==T/R lab==
 
Here is your recovery gel:
 
Here is your recovery gel:
 
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
 
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
Line 24: Line 24:
  
 
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 &mu;L of the solution that you left for us and 20 &mu;L of MW marker was loaded (for a total 1.0 &mu;g).
 
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 &mu;L of the solution that you left for us and 20 &mu;L of MW marker was loaded (for a total 1.0 &mu;g).
 +
 +
==W/F lab==
 +
 +
===Recovery gel===
 +
 +
===Pre-purification gels (for reference/posterity)===

Revision as of 12:04, 18 September 2013

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

FragsMod1.jpg
Excisedfrag.jpg


T/R lab

Here is your recovery gel: TR F1320109 M1D3 recoverygel.BMP

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

W/F lab

Recovery gel

Pre-purification gels (for reference/posterity)