Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
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(New page: === Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. === left right<br> <br style=...) |
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== Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. == | |||
[[Image:FragsMod1.jpg|left]] | [[Image:FragsMod1.jpg|left]] | ||
[[Image:Excisedfrag.jpg|right]]<br> | [[Image:Excisedfrag.jpg|right]]<br> | ||
<br style="clear:both" /> | <br style="clear:both" /> | ||
=T/R lab= | ==T/R lab== | ||
Here is your recovery gel: | Here is your recovery gel: | ||
[[Image:TR_F1320109_M1D3_recoverygel.BMP]] | [[Image:TR_F1320109_M1D3_recoverygel.BMP]] | ||
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Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | ||
==W/F lab== | |||
===Recovery gel=== | |||
===Pre-purification gels (for reference/posterity)=== | |||
Revision as of 13:04, 18 September 2013
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
T/R lab
Here is your recovery gel:
Top Gel (numbers refer to lanes):
- 1-2 Pink
- 3-4 Purple
- 5 MW
- 6-7 White
- 8-9 Blue
Bottom Gel (numbers refer to lanes):
- 1-2 Orange
- 3-4 Yellow
- 5 MW
- 6-7 Green
- 8-9 Platinum
- 10 Red
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
