Difference between revisions of "Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis"

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(And here's the gel with your recovered fragments/backbones, ready for ligation.)
(You can upload the pre-purification gels for reference here)
 
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|Green  
 
|Green  
 
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|-
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|[[Image:KU000638.BMP|200px]]
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|[[Image:OrangeTRgel bkbinsert.BMP|200px]]
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|[[Image:KU000634.BMP|200px]]
 
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=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 
[[Image:TR Row1.jpg]]
 
[[Image:TR Row1.jpg]]
 +
 +
Row 1 legend:
 +
#Red backbone
 +
#Red fragment
 +
#1 kb ladder
 +
#Orange backbone
 +
#Orange fragment
 +
#Yellow backbone
 +
#Yellow fragment
 +
#1 kb ladder
 +
#Green backbone
 +
#Green fragment
 +
 +
[[Image:TR Row2.jpg]]
 +
 +
Row 2 legend:
 +
#Blue backbone
 +
#Blue fragment
 +
#1 kb ladder
 +
#Pink backbone
 +
#Pink fragment
 +
#1 kb ladder
 +
#Purple backbone
  
 
=W/F Lab=
 
=W/F Lab=
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=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 +
 +
[[Image:WF Row1.jpg]]
 +
 +
WF Legend:
 +
#Green backbone
 +
#Green fragment
 +
#1 kb ladder
 +
#Blue backbone
 +
#Blue fragment
 +
#Pink backbone
 +
#Pink fragment
 +
#1 kb ladder
 +
#Purple backbone
 +
#Purple fragment
 +
<br style="clear:both;"/>
  
 
===You can upload the pre-purification gels for reference here===
 
===You can upload the pre-purification gels for reference here===
 +
 +
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
 +
 +
[[Image:F12_M1D3_WF-Grn.jpg|250px|thumb|left| Green Group]]
 +
[[Image:F12_M1D3_WF-Blu.jpg|250px|thumb|center| Blue Group]]
 +
[[Image:F12_M1D3_WF-Pnk.jpg|250px|thumb|left| Pink Group]]
 +
[[Image:F12_M1D3_WF-Prp.jpg|250px|thumb|center| Purple Group]]
 +
[[Image:F12_M1D3_TR_PNK.jpg|250px|thumb|center|PINK Group]]

Latest revision as of 17:21, 25 September 2012

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.

FragsMod1.jpg
Excisedfrag.jpg


T/R lab

Red Orange Yellow Green
KU000638.BMP OrangeTRgel bkbinsert.BMP KU000634.BMP
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

TR Row1.jpg

Row 1 legend:

  1. Red backbone
  2. Red fragment
  3. 1 kb ladder
  4. Orange backbone
  5. Orange fragment
  6. Yellow backbone
  7. Yellow fragment
  8. 1 kb ladder
  9. Green backbone
  10. Green fragment

TR Row2.jpg

Row 2 legend:

  1. Blue backbone
  2. Blue fragment
  3. 1 kb ladder
  4. Pink backbone
  5. Pink fragment
  6. 1 kb ladder
  7. Purple backbone

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

WF Row1.jpg

WF Legend:

  1. Green backbone
  2. Green fragment
  3. 1 kb ladder
  4. Blue backbone
  5. Blue fragment
  6. Pink backbone
  7. Pink fragment
  8. 1 kb ladder
  9. Purple backbone
  10. Purple fragment


You can upload the pre-purification gels for reference here

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Green Group
Blue Group
Error creating thumbnail: File with dimensions greater than 12.5 MP
Pink Group
Purple Group
PINK Group