Difference between revisions of "Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis"

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(And here's the gel with your recovered fragments/backbones, ready for ligation.)
(And here's the gel with your recovered fragments/backbones, ready for ligation.)
Line 32: Line 32:
 
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
 
[[Image:TR Row1.jpg]]
 
[[Image:TR Row1.jpg]]
Row 1 legend is
+
 
 +
Row 1 legend:
 
#Red backbone
 
#Red backbone
 
#Red fragment
 
#Red fragment
Line 45: Line 46:
  
 
[[Image:TR Row2.jpg]]
 
[[Image:TR Row2.jpg]]
 +
 
Row 2 legend:  
 
Row 2 legend:  
 
#Blue backbone
 
#Blue backbone

Revision as of 15:20, 18 September 2012

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.

FragsMod1.jpg
Excisedfrag.jpg


T/R lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

TR Row1.jpg

Row 1 legend:

  1. Red backbone
  2. Red fragment
  3. 1 kb ladder
  4. Orange backbone
  5. Orange fragment
  6. Yellow backbone
  7. Yellow fragment
  8. 1 kb ladder
  9. Green backbone
  10. Green fragment

TR Row2.jpg

Row 2 legend:

  1. Blue backbone
  2. Blue fragment
  3. 1 kb ladder
  4. Pink backbone
  5. Pink fragment
  6. 1 kb ladder
  7. Purple backbone

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

You can upload the pre-purification gels for reference here