Talk:20.109(F10): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
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=== And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA=== | === And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA=== | ||
Orange Team: In regards to your missing backbone, you might have too little backbone in the beginning or you've mistakenly discarded your backbone during gel purification process. Don't worry, you can certainly ask your neighbors or another group for some of their backbone to do ligation in the next lab. There should be plenty to use for everyone! | |||
[[Image:100921Gel1.JPG]] | [[Image:100921Gel1.JPG]] | ||
Revision as of 10:16, 22 September 2010
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your lab report if you so choose.
T/R lab
| Red | Orange | Yellow | Green |
| Blue | Pink | Purple | Grey |
And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA
Orange Team: In regards to your missing backbone, you might have too little backbone in the beginning or you've mistakenly discarded your backbone during gel purification process. Don't worry, you can certainly ask your neighbors or another group for some of their backbone to do ligation in the next lab. There should be plenty to use for everyone!
