Difference between revisions of "Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture"
|Line 93:||Line 93:|
Revision as of 19:55, 30 September 2008
T/R Sample Gel
Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours.
|Lane||Plasmid||sample||volume to load|
|2||cut with BamHI||20 μL|
|3||cut with XhoI||20 μL|
|4||cut with BamHI and XhoI||20 μL|
|5||1 kb marker||10 μL|
|6||candidate 1||uncut||20 μL|
|7||cut with BamHI||20 μL|
|8||cut with XhoI||20 μL|
|9||cut with BamHI and XhoI||20 μL|
|10||candidate 2||uncut||20 μL|
|11||cut with BamHI||20 μL|
|12||cut with XhoI||20 μL|
|13||cut with BamHI and XhoI||20 μL|
Please fill in which two enzymes you used in the following table.
You can download your gel images below. If the images do not show up properly in your browser, just click on the place where the image is supposed to be, and it should be visible on a new page.
Please also see the T/R sample gel above if your gel experiment did not work as you had hoped - that is, if your bands are too faint to decipher, if one or both of your enzymes didn't cut at all, or if none of your clones gave the expected product. In this case, you should analyze both your own data and the sample data in your report.
|Team colour||Digest enzyme 1||Digest enzyme 2|