T broth

From OpenWetWare
Revision as of 14:12, 11 March 2008 by Torsten Waldminghaus (talk | contribs) (added tags)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Summary

T-Broth (not to be confused with Terrific broth)

Ingredients

  1. 10 g Bacto-tryptone
  2. 5 g NaCl

Protocol

  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.

Source

Studier, FW Virology 39:562 (1969) Link: Pubmed