T broth

From OpenWetWare
Revision as of 10:40, 12 September 2005 by Skosuri (talk | contribs) (Source)

Summary

T-Broth (not to be confused with Terrific broth)

Ingredients

  1. 10 g Bacto-tryptone
  2. 5 g NaCl

Protocol

  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.

Source

Studier, FW Virology 39:562 (1969) Link: Pubmed