Difference between revisions of "TSS"

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Store at 4˚C or -20˚C.
Store at 4˚C or -20˚C.
[[Category:E.Coli Protocol]]

Revision as of 14:46, 5 December 2006


TSS buffer is used to make chemically competent cells.


To make 50 mL:

  • 5g PEG 8000
  • 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
  • 2.5 mL DMSO
  • Add LB to 50 mL

Filter sterilize (0.22 μm filter)


Store at 4˚C or -20˚C.