TSS: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
Barry Canton (talk | contribs) No edit summary |
||
| Line 3: | Line 3: | ||
*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0) | *1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0) | ||
*2.5 mL DMSO | *2.5 mL DMSO | ||
*LB to 50 mL | *Add LB to 50 mL | ||
Filter sterilize (0.22 μm filter) and store at 4˚C or -20˚C. | |||
Revision as of 10:13, 16 January 2006
To make 50 mL:
- 5g PEG 8000
- 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
- 2.5 mL DMSO
- Add LB to 50 mL
Filter sterilize (0.22 μm filter) and store at 4˚C or -20˚C.
