Difference between revisions of "TBS"

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(Recipe)
 
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*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again  
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*Prepare and sterilize filter. Store at 4°C. After diluting the 5x to 1x filter again  
  
*'''TBST''': For some applications one adds Tween 20 to TBS which makes TBST.  
+
*'''[[TBST]]''': For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.  
 
**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
 
**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
 +
**Usual concentration of Triton X-100 in TBST is 0.1%.
  
  

Latest revision as of 16:19, 10 December 2012

Purpose

  • TBS stands for tris-buffered saline and is used in many protocols for washing


Recipe

  • Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
5xTBS
for 1 liter with stock solutions
100 ml 1M Tris-HCl pH 7.5
150 ml 5M NaCl
750 ml ddH2O
  • Prepare and sterilize filter. Store at 4°C. After diluting the 5x to 1x filter again
  • TBST: For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
    • Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
    • Usual concentration of Triton X-100 in TBST is 0.1%.