TBE: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 1: | Line 1: | ||
==Purpose== | |||
*TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels. | |||
==Recipe== | |||
'''10x TBE (1 liter):''' | |||
#Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water. | #Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water. | ||
#Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) | #Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) (alternatively use 9.3 g Na<sub>2</sub>EDTA) | ||
#Adjust volume to 1 Liter. | |||
#Store at room temperature. | |||
'''1x TBE (1 liter):''' | |||
#Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water. | |||
#Add 4 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) | |||
#Adjust volume to 1 Liter. | #Adjust volume to 1 Liter. | ||
#Store at room temperature. | #Store at room temperature. | ||
Revision as of 00:06, 7 May 2008
Purpose
- TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
Recipe
10x TBE (1 liter):
- Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
- Add 40 ml 0.5 M Na2EDTA (pH 8.0) (alternatively use 9.3 g Na2EDTA)
- Adjust volume to 1 Liter.
- Store at room temperature.
1x TBE (1 liter):
- Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
- Add 4 ml 0.5 M Na2EDTA (pH 8.0)
- Adjust volume to 1 Liter.
- Store at room temperature.
Commercial sources
- UltraPure™ 10X TBE Buffer from Invitrogen
